From ovarian stimulation to insemination of oocytes: A travel in time?

The moment that the sperm enters the egg is crucial for the development of the embryo. Nowadays, we know that in order to achieve successful fertilization and optimal embryo development, it is necessary to have an oocyte with both nuclear and cytoplasmic maturity, as this ensures that many important developmental processes take place. Early entry of the sperm into the egg may lead to non-fertilization or to an embryo with slow development.

The aim of the study, that took place in Embryolab, was to determine the time range after ovulation induction, which ensures optimal egg maturation potential, as well as higher fertilization and pregnancy rates. The study showed that the time range that resulted in the highest fertilization rates and optimal embryo development is 38.5 hours.

Larger numbers of mature and fertilized oocytes lead to more available embryos per case, which increases the overall chance of pregnancy and child birth.

The poster presented by Mrs. Mary Karagianni B.Sc., M.Sc. Biologist, Clinical Embryologist, at the 35th annual conference of ESHRE

 Vitrification of day 4 embryos: Our new tool for infertility treatment?

Vitrification of embryos is a highly successful method, which guarantees survival of embryos up to almost 100%! Vitrification has become an integral part of the in vitro fertilization (IVF) practices since 2005 and has contributed significantly to the increased success rates after IVF. Up to date, the vast majority of our published data involves vitrification of day 3 (5-8 cells) or day 5 embryos (blastocysts). There are very few data on the survival and implantation rates of embryos vitrified on day 4 of their development (morula).

Our unit has implemented vitrification of day 4 embryos since many years and is presenting the data at the annual meeting of the European Society of Human Reproduction (ESHRE) in Vienna. According to the study vitrification of day 4 embryos is equally effective and when combined with day 5 embryo transfer the pregnancy rates are significantly increased.

The poster presented by Mrs. Alexia Chatziparasidou M.Sc., Sr. Clinical Embryologist, Embryolab Lab Director, Embryolab & Academy Co-Founder, at the 35th annual conference of ESHRE

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