Course #9
DFI – Sperm DNA Fragmentation

Oocyte collection and cumulus removal

About the course:

The course provides a one to one hands-on training experience. Experts with long experience in DFI testing will teach you the practical aspects of DNA fragmentation assay.


This one-day intense one to one hands-on training course was designed to introduce junior scientists, technicians and andrologists into the practical aspects of DNA fragmentation test. 

Course’s goals:

After this course the candidate will be able to:

  • Prepare the setting for the DFI test
  • Perform the steps of the HALO technique
  • Assess the Halo test
  • Report the DFI result

Training Material: 

  • Donor sperm
  • Training Videos


9-12: The Sperm Expert Introduces you to the:

  • Fundamentals of the HALOSperm test
  • Criteria for the Halo technique
  • Essential Steps for the HALO technique
  • Tips and tricks for the HALO technique
  • Samples Interpretation & Reporting
  • QC and QA of the Halo technique

 12:00-13:00: Lunch Break

13:00-17:00: Hands on Session with the Sperm Expert

  • Prepare ejaculate sample for the Halo technique
  • Halo technique in practice
  • Microscopic evaluation of the treated samples
  • Tips and Tricks for the Halo Technique

Cost: 700 euros

Certificate of attendance: 

All candidates will receive a certificate of attendance upon submitting the course’s evaluation form.

TEL: +30.2310.473000

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